Discovery Biology - Assay Development and HTS

InSymbiosis has a wealth of experience in early phase drug discovery. Our expertise in assay development and high-throughput screening (HTS) enables us to take discovery projects from the cloning of the target through HTS into lead optimization.

Drug Target Cloning and Expression

Cloning and expression in bacterial, yeast and mammalian systems
Custom cell line generation and characterization
Protein purification and characterization
Large scale protein production
Cell culture scale up and membrane production

Assay Development

Biochemical:
- Direct enzyme activity (kinase, phosphatase, protease)
- Immunoassays (ELISA and ELISA conversions)
- Receptor binding (GPCRs, RTKs, ion channels)
- Protein-protein interactions
- Biacore (SPR) technology (label-free study of biomolecular interactions)
Cell based assays:
- Second messenger (e.g. cAMP) for GPCRs
- Patch clamp electrophysiology for ion channels
- Gene reporter
- Cell proliferation, viability, cytotoxicty
- Apoptosis
- Cell motility, invasion
- FACS methods

High Throughput Screening

Automated Planar Patch Clamp For Ion Channels

Pre-validated (e.g. hERG) and custom assays available

Compound Libraries

Commercial library, collection of plant extracts, synthetic & commercial compounds, and known drug molecules
Design and generation of small molecule libraries and peptides/peptide mimetics

Please see our Drug Discovery brochure for more details or Contact Us.