Drug Discovery

Discovery ADME, Pharmacokinetic and Early Toxicology

InSymbiosis offers a range of key in vitro ADME assays as well as in vivo pharmacokinetic studies to support our clients’ early phase discovery programs so that they can move forward to later preclinical and clinical development with greater confidence and diminish the ever-increasing cost of bringing drugs to market.

ADME

  • In vitro Metabolism
  • Drug permeability: Caco-2 assay
  • Plasma Stability Studies
  • Plasma Protein Binding Studies
  • Solubility, logP, Pka and LogD analysis

Pharmacokinetics

InSymbiosis has the capacity to meet our client’s needs in early pharmacokinetic screening in our AAALAC and CCAC compliant full animal care facility.

Bioanalytical Chemistry

At InSymbiosis, our analytical laboratory performs routine analytical testing, specialized analytical method development and validation as well as exploratory research programs (optional GLP & GMP compliance).

  • Development, optimization, evaluation & validation of analytical methods
  • Cleaning validation method development and validation
  • Identification, isolation & purification of impurities/degradation products (HPLC semi-prep & commercial prep)
  • Official testing based on USP and EP monograph

Early Toxicology Studies

The assessment of toxicological parameters is becoming more necessary in early drug discovery in order to reduce the attrition rate at later phases of drug development.

InSymbiosis provides a range of in vitro and in vivo

  • Cytotoxicity and cell viability assays
  • Cardiac toxicology (available in human cardiac tissue)
    • hERG manual whole-cell patch clamp assay and automated planar patch system
    • Action potential kinetics analysis in Purkinje fibres or contractile cardiac tissue
    • Cardiac and vascular Muscle Tension Measurement
    • Excitation-Contraction Coupling assay
    • Transmural Dispersion of Repolarization assay
  • Bacterial reverse mutation-AMES Test
  • In vivo acute toxicity studies in rodents
    • Whole body, diagnostic, and system/organ specific necropsy
    • X-rays, biochemistry, hematology, microbiology, parasitology and serology
    • Expert veterinary pathologist interpretation of data
    • Histology and pathology services available

Please see our Drug Discovery brochure for more details or Contact Us.